Would like to obtain more info and learn more of its applications?
Massive Photonics
Massive Photonics
Massive Photonics is a company focusing on providing DNA PAINT technologies to customers who are working in the field of single molecular localization microscopy (SMLM), like STORM, dSTORM/ONI, PALM, MINFLUX, etc. PTC is the sole distributor of Massive Photonics in Hong Kong and Macau to market their products.
Overview of DNA-PAINT technology
WHAT IS DNA-PAINT?
Massive Photonics uses short, dye-labelled DNA strands that transiently bind to their complements, conjugated to affinity reagents for protein detection. The resulting target “blinking” yields high-resolution image
WHY USE DNA-PAINT?
- Ultra resolution! Resolve 10 – 15 nm distances in cells.
- Unlimited target multiplexing! In DNA-PAINT, spectrally compatible dyes aren’t needed to image multiple targets at a time. Instead, you can simply couple different docking strands (e. different DNA sequences) to your target binders and image each target sequentially using the same laser line with its corresponding imager strand.
- Target Counting! The predictable nature of DNA binding kinetics allows you to quantify how many proteins are present in cells (qPAINT).
WHERE TO START?
You need 3 things to do DNA-PAINT:
- Simple TIRF Microscope – like the ZEISS Elyra 7, Nikon N-STORM, ONI Nanoimager, abbelight SAFe 180, Bruker VUTARA VXL microscope, Andor Dragonfly or similar system found in your imaging facility. Advanced Microscope – MINFLUX.
- DNA-PAINT compatible binder – any antibody or single-domain antibody that binds to your target protein and is coupled to a docking strand. Find a DNA-PAINT compatible binder here at Massive Photonics! We have a wide range of antibodies and single domain antibodies. Order your custom antibody or single-domain antibody (PRODUCTS page).
- Picasso data analysis software – a free software developed for DNA-PAINT image analysis (Download Picasso at Github). Find out how to use Picasso in Nature Protocols 12, 1198–1228 (2017)
Suitable applications
DNA-PAINT 580 Optimized For MINFLUX
DNA-PAINT 660 Optimized For MINFLUX
DNA-PAINT FAST-DNA-PAINT kit for 1 plex 2 plex 3 plex
DNA-PAINT FAST-DNA-PAINT kit for tag protein (e.g. Halo, GFP, RFP, ALFA, etc.)
DNA-PAINT kit for direct labelling of neuron protein
DNA-PAINT kit for direct labelling of immune protein
DNA-PAINT kit for direct labelling of actin protein
DNA-PAINT kit for ONI
- RNA FISH
RNA-FISH kit for GAPDHNeat1TFRCMalat1
RNA-FISH custom kit
- Multiplexing
DNA-PAINT kit for multiplexing (up to 6 plex)
- RESI kit
RESI kit anti-ALFA
RESI kit anti-GFP
Massive Photonics
Massive Photonics is a company focusing on providing DNA PAINT technologies to customers who are working in the field of single molecular localization microscopy (SMLM), like STORM, dSTORM/ONI, PALM, MINFLUX, etc. PTC is the sole distributor of Massive Photonics in Hong Kong and Macau to market their products.
Overview of DNA-PAINT technology
WHAT IS DNA-PAINT?
Massive Photonics uses short, dye-labelled DNA strands that transiently bind to their complements, conjugated to affinity reagents for protein detection. The resulting target “blinking” yields high-resolution image
WHY USE DNA-PAINT?
- Ultra resolution! Resolve 10 – 15 nm distances in cells.
- Unlimited target multiplexing! In DNA-PAINT, spectrally compatible dyes aren’t needed to image multiple targets at a time. Instead, you can simply couple different docking strands (e. different DNA sequences) to your target binders and image each target sequentially using the same laser line with its corresponding imager strand.
- Target Counting! The predictable nature of DNA binding kinetics allows you to quantify how many proteins are present in cells (qPAINT).
WHERE TO START?
You need 3 things to do DNA-PAINT:
- Simple TIRF Microscope – like the ZEISS Elyra 7, Nikon N-STORM, ONI Nanoimager, abbelight SAFe 180, Bruker VUTARA VXL microscope, Andor Dragonfly or similar system found in your imaging facility. Advanced Microscope – MINFLUX.
- DNA-PAINT compatible binder – any antibody or single-domain antibody that binds to your target protein and is coupled to a docking strand. Find a DNA-PAINT compatible binder here at Massive Photonics! We have a wide range of antibodies and single domain antibodies. Order your custom antibody or single-domain antibody (PRODUCTS page).
- Picasso data analysis software – a free software developed for DNA-PAINT image analysis (Download Picasso at Github). Find out how to use Picasso in Nature Protocols 12, 1198–1228 (2017)
Suitable applications
DNA-PAINT 580 Optimized For MINFLUX
DNA-PAINT 660 Optimized For MINFLUX
DNA-PAINT FAST-DNA-PAINT kit for 1 plex 2 plex 3 plex
DNA-PAINT FAST-DNA-PAINT kit for tag protein (e.g. Halo, GFP, RFP, ALFA, etc.)
DNA-PAINT kit for direct labelling of neuron protein
DNA-PAINT kit for direct labelling of immune protein
DNA-PAINT kit for direct labelling of actin protein
DNA-PAINT kit for ONI
- RNA FISH
RNA-FISH kit for GAPDHNeat1TFRCMalat1
RNA-FISH custom kit
- Multiplexing
DNA-PAINT kit for multiplexing (up to 6 plex)
- RESI kit
RESI kit anti-ALFA
RESI kit anti-GFP
Would like to obtain more info and learn more of its applications?