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µ-Slide 8 Well high Glass Bottom
A chambered coverslip with 8 individual wells, high walls, and a #1.5H glass coverslip bottom, suitable for use in TIRF and single molecule applications
- A cell culture chamber for observation of the sample through a glass coverslip bottom using high resolution microscopy
- Brilliant cell imaging thanks to the low thickness variability of the coverslips’ glass
- Cost-effective experiments using small numbers of cells and low volumes of reagents
- Extra high individual walls to keep cross contamination between wells as low as possible
Applications
- Cultivation and high-resolution microscopy of cells
- TIRF and single molecule applications of living and fixed cells
- Super-resolution microscopy
- Immunofluorescence staining and fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Transfection assays
- Differential interference contrast (DIC) microscopy when used with a DIC lid
Specifications
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 8 |
| Dimensions of wells (w x l x h) | 9.4 x 10.7 x 9.3 mm³ |
| Volume per well | 300 µl |
| Total height with/without lid | 10.8/9.5 mm |
| Growth area per well | 1.0 cm² |
| Coating area per well | 2.2 cm² |
| Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm | |
Technical Features
- Chambered coverslip with 8 independent wells and a non-removable glass coverslip-bottom
- Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
- May require coating to promote cell attachment
- Individual well walls for minimizing well-to-well crosstalk and contaminations
- Compatible with staining and fixation solutions
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