Applications
- Generation and long-term culture of 3D cell aggregates (e.g., spheroids and organoids) without any scaffold
- High resolution fluorescence microscopy of organoids, spheroids, embryoid bodies (EBs), and cells in suspension
- Background-free analysis of cell-cell interactions
- Prevention of stem cell differentiation due to attachment
- Culture of suspension cells in a permanently unattached state
- Self-assembly tumor spheroid formation assays
- 3D tumor spheroid models
Specifications
Bioinert Surface
Bioinert surface thickness | 200 nm |
Bioinert surface material | Polyol-based hydrogel |
Protein coatings | Not possible |
µ-Slide 4 Well
Outer dimensions (w x l) | 25.5 x 75.5 mm² |
Number of wells | 8 |
Dimensions of wells (w x l x h) | 9.4 x 10.7 x 9.3 mm³ |
Volume per well | 300 µl |
Total height with / without lid | 10.8 mm / 9.5 mm |
Growth area per well | 1.0 cm² |
Coating area per well | 2.2 cm² |
Bottom: ibidi Polymer Coverslip with Bioinert surface |
Technical Features
- Chambered coverslip with 8 independent wells and a non-removable polymer coverslip-bottom
- µ-Slide 4 Well with a non-adherent, passivated Bioinert surface
- Bioinert surface with long term passivation—superior to standard ULA surfaces:
- Ready-to-use without prior surface treatment or preparation
- No adsorption, coating, or binding of proteins, antibodies, enzymes, and other biomolecules
- Non-cytotoxic, biologically inert, and non-degradable
- Imaging chamber slide with excellent optical quality for high-end microscopy
- Individual well walls for minimizing well-to-well crosstalk and contaminations
- Compatible with staining and fixation solutions
- Biocompatible polymer material—no glue, no leaking