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Culture-Insert family
Culture-Insert family
Explore the Culture-Insert family here
Applications
- Wound healing and migration assays
- 2D invasion assays
- Co-cultivation
Features
- Reproducible experiments
- a defined 500 µm cell-free gap
- no leakage during cultivation
- no material being left behind after the insert’s removal
- Available in 35mm u-Dish and 24 well plate - ready to use; and culture insert for self-insertion
Movie
- Performing a Wound Healing Assay
- Automated Image Analysis with ibidi ACAS online software (movie or time lapse photos can be used)
Principle for Wound Healing and Migration Assays
Culture-Inserts vs. Scratch Assay
ibidi Culture-Inserts | Scratch assay | |
|---|---|---|
Gap creation | Cell seeding into designated areas | Scratching the cell surface with a needle or pipet tip |
Gap size | Defined | Varying (i.e, not reproducible) |
Gap surface residues | No | Extracellular matrix residues possible |
Vessel surface damage | No | Yes | due to mechanical scratching on the surface |
Cell damage | No | Yes | due to scratching the cells |
Segregation & signaling of necrotic/apoptotic cells | Low | High |
Internal reference* | Yes | No |
Example: Sample result of an ACAS output of wound healing assay
Culture-Insert family
Explore the Culture-Insert family here
Applications
- Wound healing and migration assays
- 2D invasion assays
- Co-cultivation
Features
- Reproducible experiments
- a defined 500 µm cell-free gap
- no leakage during cultivation
- no material being left behind after the insert’s removal
- Available in 35mm u-Dish and 24 well plate - ready to use; and culture insert for self-insertion
Movie
- Performing a Wound Healing Assay
- Automated Image Analysis with ibidi ACAS online software (movie or time lapse photos can be used)
Principle for Wound Healing and Migration Assays
Culture-Inserts vs. Scratch Assay
ibidi Culture-Inserts | Scratch assay | |
|---|---|---|
Gap creation | Cell seeding into designated areas | Scratching the cell surface with a needle or pipet tip |
Gap size | Defined | Varying (i.e, not reproducible) |
Gap surface residues | No | Extracellular matrix residues possible |
Vessel surface damage | No | Yes | due to mechanical scratching on the surface |
Cell damage | No | Yes | due to scratching the cells |
Segregation & signaling of necrotic/apoptotic cells | Low | High |
Internal reference* | Yes | No |
Example: Sample result of an ACAS output of wound healing assay
Would like to obtain more info and learn more of its applications?