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µ-Slide 18 Well high Glass Bottom
A chambered glass coverslip with 18 wells for cell culture, suitable for use in TIRF microscopy and single molecule applications
- All-in-one 18 well chamber slide for cost-effective experiments with small cell numbers and low reagent volumes
- Microscopy slide that is ideal for high-resolution imaging through the No. 1.5H glass bottom with the highest optical quality and low thickness variability
- Cell culture chamber suitable for most microscopy techniques (e.g., TIRF, super-resolution microscopy, DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, and LSFM)
Applications
- Cultivation and high-resolution microscopy of cells
- TIRF and single molecule applications of living and fixed cells
- Super-resolution microscopy
- Immunofluorescence staining and fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Transfection assays
- Differential interference contrast (DIC) microscopy when used with a DIC lid
Specifications
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 18 |
| Dimensions of wells (w x l x h) | 5.7 x 6.1 x 6.8 mm³ |
| Volume per well | 100 µl |
| Height with/without lid | 8.2/6.8 mm |
| Growth area per well | 0.34 cm² |
| Coating area per well | 1.15 cm² |
| Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm | |
Technical Features
- Chambered glass coverslip with 18 independent wells
- Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
- May require coating to promote cell attachment
- Imaging chamber slide with excellent optical quality for high-end microscopy
- Closely fitting lid for low evaporation
- Individual well walls for minimizing well-to-well crosstalk and contaminations
- Compatible with staining and fixation solutions
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